Fig 2: Cell localization of CXCL12 and CXCR4 in spinal dorsal horn of SNL rats. Double staining images A,B, and C showed that CXCL12 (red) is colocalized with NeuN (green, right), but not with GFAP (green, left) or OX42 (green, middle). while CXCR4 (red) is colocalized with GFAP and NeuN, but not with OX42, as shown in D, E, and F. More details are in A-1 to F-1.Spinal segments were collected 7 d after SNL (all images, scale bar, 100 µm)

Fig 3: SDF-1 inhibits the dedifferentiation of islet ß cells after it binds to CXCR4 to form a complex. (A) Before and after 48 h of intervention with DPP-IV of different concentrations in Min6 cells, the results of Western blot and relative expression value results (B) (n = 3). ***p < 0.001. (C) Western blot results of Min6 cells before and after intervention with DPP-IV, and relative expression value results (D) (n = 3). (E) The results of CO-IP and relative expression value results (F) (SDF-1 relative to CXCR4) before and after DPP-IV intervention in Min6 cells (n = 3). *p < 0.05, **p < 0.01, ***p < 0.001

Fig 4: CXCR4 overexpression promoted tumor growth of HCC in mice via the c‐Met signaling pathway. (A) The tumor xenograft mouse model was constructed by subcutaneous injection of the normal or transfected Huh7 cells and received administration of gefitinib. The weight (B) and volume (C) of tumor in mice were measured. n = 4. One‐way ANOVA. Data were presented as mean ± standard deviation. **P < 0.01 versus normal; ## P < 0.01 versus LV‐CXCR4.

Fig 5: CXCR4/mTOR signaling and ABAT gene expression analysis in cell lines and clinical samples.A ABAT gene expression was analyzed in the GSE19429 dataset. B Expression correlation analysis between ABAT and interferon response-related genes in the GSE19429 dataset. Relative expression of CXCR4, IRF7, IRF9, IRS1, IFNAR1, and IFNGR1 gene in ABAT silenced THP-1 (C) and HL-60 (D) cells. E Correlation analysis of ABAT and CXCR4 gene in MDS samples. F Protein expressions of p-mTOR, mTOR, and CXCR4 in THP-1 and HL-60 cells with ABAT knockdown and HMAs treatment. G CXCR4 gene expression after ABAT overexpression. H IRF7, IRF9, IRS1, IFNAR1, and IFNGR1 gene expressions in the above two cell lines with ABAT overexpression. I Protein expression of p-mTOR, mTOR, and CXCR4 in THP-1 and HL-60 cells after ABAT overexpression and HMAs treatment. Data were represented as mean ± SEM from three biological samples. *P < 0.05, **P < 0.01, ***P < 0.001. Pearson correlation analysis, Student’s t-test (two groups), and one-way ANOVA analysis followed by Dunnett’s test (multiple groups). ns. not significant.